AceShowbiz - The pop superstar Lady GaGa's acceptance anthem "Born This Way" became the theme of the show in an installment broadcast in the U.S. on Tuesday.

The cast all wore t-shirts proclaiming their characters' insecurities, with Matthew Morrison donning a shirt emblazoned with the phrase "Butt Chin," while Chris Colfer's gay character proclaims across his chest "Likes Boys".

The storyline drew some criticism from conservative campaigners in the U.S. for its pro-gay themes, but outspoken activist Lady GaGa has given the episode her full backing, writing in a post on her Twitter page, "I really loved Glee's 'Born This Way' episode. I admire the show for being brave + fighting for such modern social messaging. Never back down."

Jarrett Barrios, president of Gay & Lesbian Alliance Against Defamation (GLAAD), has also staunchly defended the TV musical, telling the Hollywood Reporter, "Fair-minded Americans are tuning in by the millions to inclusive shows like 'Glee'... because they don't care whether someone is straight or gay - what they care about is seeing characters and stories they can relate to. Most Americans today support full equality for their gay and lesbian friends, family and neighbours. That anti-gay critics continue to be out-of-touch with the majority and can't see that fact is no surprise."